Ikoreshwa rya tekinoroji yo gukonjesha ibikoresho bya PCR

Ikoreshwa rya tekinoroji yo gukonjesha ibikoresho bya PCR

Ikoreshwa rya tekinoroji yo gukonjesha mu bikoresho bya PCR ahanini biri mu kugenzura ubushyuhe. Inyungu yibanze nubushobozi bwihuse kandi busobanutse bwo kugenzura ubushyuhe, butanga intsinzi yubushakashatsi bwa ADN bwongerewe imbaraga.

Ibyingenzi byingenzi

1. Kugenzura neza ubushyuhe

Igikoresho cya PCR gikeneye kuzenguruka mu byiciro bitatu: gutandukanya ubushyuhe bwo hejuru (90-95 ℃), ubushyuhe bwo hasi (55-65 ℃), no kongera ubushyuhe bwiza (70-75 ℃). Uburyo bwa firigo gakondo biragoye kubahiriza ibisabwa ± 0.1 ℃. Ubukonje bwa Thermoelectric, tekinoroji yo gukonjesha igera kuri milisekondi kurwego rwo kugenzura ubushyuhe binyuze mu ngaruka za Peltier, birinda kunanirwa kwa amplification biterwa nubushyuhe bwa 2 ℃.

2. Gukonjesha vuba no gushyushya

Ubushuhe bwo gukonjesha bwa Thermoelectric, moderi yubushyuhe, ibikoresho bya peltier, moderi ya peltier irashobora kugera ku gipimo cyo gukonja cya dogere selisiyusi 3 kugeza ku isegonda, bikagabanya cyane ukwezi kwubushakashatsi ugereranije na dogere selisiyusi 2 kumasegonda ya compressor gakondo. Kurugero, 96-iriba rya PCR ikoresha tekinoroji yo kugenzura ubushyuhe bwa zone kugirango harebwe ubushyuhe buhoraho mumwanya wose mwiza kandi wirinde itandukaniro rya 2 ℃ ubushyuhe buterwa ningaruka.

3. Kongera ubwizerwe bwibikoresho

Module ikonjesha ya thermoelectric, moderi ya peltier, peltier lements, modul ya TEC ya Beijing Huimao Cooling Equipment Co., Ltd yahindutse ibice byingenzi bigenzura ubushyuhe bwibikoresho bya PCR kubera kwizerwa kwinshi. Ingano yacyo ntoya hamwe n urusaku rudafite urusaku bituma ikenerwa neza nibisabwa mubuvuzi.

Imanza zisanzwe

96-nziza ya fluorescence igereranya PCR: Yinjijwe hamwe na moderi yo gukonjesha ya termoelektrike, module ya TEC, igikoresho cya peltier, moderi ya peltier ituma igenzura ryukuri ryubushyuhe bwikigereranyo cyinshi kandi rikoreshwa cyane mubice nko gusesengura imiterere ya gene no gutahura indwara.

Firigo zikoreshwa mubuvuzi zishobora gukonjeshwa: gukonjesha amashanyarazi, gukonjesha peltier gukonjesha kwa firigo zikoreshwa mu kubika ibicuruzwa nkinkingo n’imiti bisaba ibidukikije bidafite ubushyuhe buke, bigatuma ubushyuhe butajegajega mugihe cyo gutwara.

Ibikoresho byo kuvura lazeri:

Moderi yo gukonjesha ya Thermoelectric, peltier element, moderi ya thermoelectric ikonjesha emitter ya laser kugirango igabanye ibyago byo gutwika uruhu no kongera umutekano wubuvuzi.

TEC1-39109T200 Ibisobanuro

Ubushyuhe bwo ku ruhande bushyushye ni 30 C,

Imax ： 9A

Umax: 46V

Qmax ： 246.3W

ACR: 4 ± 0.1Ω（ Ta = 23 C）

Delta T max ： 67 -69C

Ingano ： 55x55x3.5-3.6mm

TES1-15809T200 Ibisobanuro

Ubushyuhe bwo ku ruhande bushyushye: 30 C,

Imax ： 9.2A ，

Umax: 18.6V

Qmax ： 99.5 W.

Delta T max ： 67 C.

ACR ： 1.7 ± 15% Ω （1.53 kugeza 1.87 Ohm）

Ingano ： 77 × 16.8 × 2.8mm

Umugozi: 18 AWG silicone wire cyangwa Sn-isa hejuru hejuru, ubushyuhe bwo hejuru Kurwanya 200 ℃