Gukonjesha kwa Peltier (tekinoroji yo gukonjesha ya thermoelectric ishingiye ku ngaruka za Peltier) yabaye imwe mu buhanga bwibanze bwa sisitemu yo kugenzura ubushyuhe bwibikoresho bya PCR (polymerase chain reaction) bitewe nuburyo bwihuse bwihuse, kugenzura ubushyuhe bwuzuye, hamwe nubunini buke, bigira ingaruka zikomeye kumikorere ya PCR. Ibikurikira nisesengura rirambuye ryibisobanuro byihariye hamwe nibyiza byo gukonjesha amashanyarazi (gukonjesha peltier) guhera kubisabwa byibanze bya PCR:
I. Ibisabwa Byibanze Kugenzura Ubushyuhe muri Tekinoroji ya PCR
Inzira yibanze ya PCR ninzinguzingo isubiramo yo gutandukana (90-95 ℃), annealing (50-60 ℃), no kwaguka (72 ℃), ifite ibisabwa cyane kuri sisitemu yo kugenzura ubushyuhe.
Ubushyuhe bwihuse kuzamuka no kugwa: Gabanya igihe cyumuzenguruko umwe (kurugero, bisaba amasegonda make kugirango ugabanuke uva kuri 95 ℃ ujya kuri 55 ℃), kandi byongere imbaraga mubikorwa;
Kugenzura ubushyuhe-busobanutse neza: Gutandukana ± 0.5 ℃ mu bushyuhe bwa annealing birashobora gutuma umuntu adashobora kwiyongera, kandi bigomba kugenzurwa muri ± 0.1 ℃.
Ubushyuhe bumwe: Iyo ingero nyinshi zifata icyarimwe, itandukaniro ryubushyuhe hagati yicyitegererezo Iriba igomba kuba ≤0.5 ℃ kugirango wirinde gutandukana.
Guhindura imihindagurikire ya Miniaturisiyasi: PCR igendanwa (nko gupima ibibanza POCT yerekana) igomba kuba yuzuye mubunini kandi idafite ibice byo kwambara.
II. Porogaramu Zibanze zo gukonjesha amashanyarazi muri PCR
Thermoelectric Cooler TEC, Moderi yo gukonjesha ya Thermoelectric, module ya peltier igera kuri "guhinduranya byerekeranye no gushyushya no gukonjesha" binyuze mumashanyarazi ataziguye, bihuye neza nibisabwa n'ubushyuhe bwa PCR. Porogaramu yihariye igaragarira mubice bikurikira:
1. Ubushyuhe bwihuse kuzamuka no kugwa: Gabanya igihe cyo kubyitwaramo
Ihame: Muguhindura icyerekezo cyubu, module ya TEC, module ya thermoelectric, igikoresho cya peltier kirashobora guhinduka byihuse hagati ya "gushyushya" (mugihe ikigezweho kiri imbere, iherezo ryogukwirakwiza ubushyuhe bwa module ya TEC, module ya peltier ihinduka iherezo ryubushyuhe) na "gukonjesha" (mugihe ikigezweho gihindutse, iherezo ryubushyuhe rihinduka iherezo ryubushuhe).
Ibyiza: Uburyo bwa firigo gakondo (nkabafana na compressor) bushingira kumashanyarazi cyangwa kugenda mumashini, kandi igipimo cyo gushyushya no gukonjesha mubisanzwe kiri munsi ya 2 ℃ / s. Iyo TEC ihujwe nubushyuhe bwo hejuru bwumuriro mwinshi (nkumuringa na aluminiyumu), irashobora kugera ku gipimo cyo gushyushya no gukonjesha cya 5-10 ℃ / s, igabanya igihe cyizunguruka cya PCR kuva muminota 30 ikagera munsi yiminota 10 (nko mubikoresho byihuse bya PCR).
2. Kugenzura ubushyuhe-busobanutse neza: Kugenzura umwihariko wa amplification
Ihame: Imbaraga zisohoka (gushyushya / gukonjesha ubukana) bwa moderi ya TEC, module yo gukonjesha ya termoelektrike, module ya thermoelectric module ihujwe neza nimbaraga zubu. Uhujije hamwe nubushyuhe bwo hejuru cyane (nka platine irwanya platine, thermocouple) hamwe na sisitemu yo kugenzura ibitekerezo bya PID, ikigezweho kirashobora guhinduka mugihe nyacyo kugirango igere ku bushyuhe bwuzuye.
Ibyiza: Kugenzura ubushyuhe burashobora kugera kuri ± 0.1 ℃, ibyo bikaba birenze cyane ibyo kwiyuhagira bisanzwe cyangwa gukonjesha compressor (± 0.5 ℃). Kurugero, niba ubushyuhe bwintego mugihe cya annealing ari 58 ℃, module ya TEC, module ya thermoelectric, peltier cooler, peltier element irashobora kugumana ubu bushyuhe, birinda guhuza bidasanzwe primers bitewe nihindagurika ryubushyuhe kandi bikazamura cyane umwihariko wa amplification.
3. Igishushanyo mbonera: Guteza imbere iterambere rya PCR igendanwa
Ihame: Ingano ya module ya TEC, peltier element, igikoresho cya peltier ni santimetero kare gusa (urugero, module ya 10 × 10mm ya moderi ya TEC, moderi yo gukonjesha ya termoelektrike, module ya peltier irashobora kuba yujuje ibisabwa byurugero rumwe), ntabwo ifite ibice byimashini (nka piston ya compressor cyangwa blade), kandi ntibisaba frigo.
Ibyiza: Iyo ibikoresho bya PCR gakondo bishingiye kuri compressor kugirango bikonje, ingano yabyo irenga 50L. Nyamara, ibikoresho byimukanwa bya PCR ukoresheje moderi yo gukonjesha ya termoelektrike, module ya thermoelectric, module ya peltier, module ya TEC irashobora kugabanuka kugeza munsi ya 5L (nkibikoresho bifata intoki), bigatuma ibera ibizamini byo mumurima (nko kwerekanwa kumwanya mugihe cyibyorezo), kwipimisha kuryama kwa clinique, nibindi bintu.
4. Uburinganire bwubushyuhe: Menya neza guhuza ingero zitandukanye
Ihame: Mugutegura ibice byinshi bya TEC (nka 96 micro TECs ihuye nisahani ya 96-iriba), cyangwa ufatanije nicyuma gisaranganya ubushyuhe (ibikoresho byogukoresha ubushyuhe bwinshi), gutandukana kwubushyuhe biterwa no gutandukana kwabantu muri TEC birashobora gusubirwamo.
Ibyiza: Itandukaniro ryubushyuhe buri hagati yicyitegererezo Iriba irashobora kugenzurwa muri ± 0.3 ℃, ukirinda itandukaniro ryimikorere ya amplification iterwa nubushyuhe budahuye hagati yuruzitiro rwa Iriba na Iriba rwagati, no kwemeza kugereranya ibisubizo byintangarugero (nko guhuza indangagaciro za CT mugihe nyacyo cya fluorescence PCR).
5. Kwizerwa no gukomeza: Kugabanya ibiciro byigihe kirekire
Ihame: TEC ntabwo ifite ibice byo kwambara, ifite igihe cyamasaha arenga 100.000, kandi ntisaba gusimbuza buri gihe firigo (nka Freon muri compressor).
Ibyiza: Impuzandengo yubuzima bwigikoresho cya PCR gikonjeshwa na compressor gakondo ni hafi imyaka 5 kugeza 8, mugihe sisitemu ya TEC irashobora kuyigeza kumyaka irenga 10. Byongeye kandi, kubungabunga bisaba gusa koza ubushyuhe, kugabanya cyane imikorere nigiciro cyibikoresho.
III. Inzitizi hamwe na Optimizasiyo muri Porogaramu
Gukonjesha Semiconductor ntabwo ari byiza muri PCR kandi bisaba guhitamo neza:
Ubushyuhe bwo gukwirakwiza ubushyuhe: Iyo TEC ikonje, ubushyuhe bwinshi bwirundanya kurangira ubushyuhe (urugero, iyo ubushyuhe bugabanutse kuva kuri 95 ℃ kugeza kuri 55 ℃, itandukaniro ryubushyuhe rigera kuri 40 and, kandi imbaraga zo kurekura ubushyuhe zikiyongera cyane). Birakenewe kubihuza na sisitemu yo gukwirakwiza ubushyuhe neza (nk'ubushyuhe bw'umuringa ucengera + abafana ba turbine, cyangwa moderi yo gukonjesha amazi), bitabaye ibyo bizatuma igabanuka ry'ubukonje (ndetse no kwangirika cyane).
Igenzura ry'ingufu zikoreshwa: Mu itandukaniro rinini ry'ubushyuhe, gukoresha ingufu za TEC ni hejuru cyane (urugero, imbaraga za TEC z'igikoresho 96-cyiza cya PCR gishobora kugera ku 100-200W), kandi ni ngombwa kugabanya ingufu zidakoreshwa neza binyuze muri algorithm zifite ubwenge (nko kugenzura ubushyuhe bw'ubushyuhe).
Iv. Imanza zifatika
Kugeza ubu, ibikoresho byingenzi bya PCR (cyane cyane igihe nyacyo cya fluorescence igereranya ibikoresho bya PCR) muri rusange yakoresheje tekinoroji yo gukonjesha igice, urugero:
Ibikoresho byo muri laboratoire: 96-iriba rya fluorescence igereranya PCR igikoresho runaka, kigaragaza ubushyuhe bwa TEC, hamwe nubushyuhe no gukonjesha bigera kuri 6 ℃ / s, kugenzura ubushyuhe bwa ± 0.05 and, no gushyigikira 384-neza-byinjira cyane.
Igikoresho kigendanwa: Igikoresho runaka gifashwe na PCR (gipima munsi ya 1kg), gishingiye ku gishushanyo cya TEC, kirashobora kurangiza gutahura igitabo cyitwa coronavirus mu minota 30 kandi kibereye ahantu nyaburanga nko ku bibuga by'indege no mu baturage.
Incamake
Gukonjesha kwa Thermoelectric, hamwe nibyiza bitatu byingenzi byokwitwara byihuse, bisobanutse neza na miniaturizasiya, byakemuye ingingo zingenzi zububabare bwikoranabuhanga rya PCR muburyo bwo gukora neza, umwihariko no guhuza n'imiterere, bihinduka ikoranabuhanga risanzwe ryibikoresho bya PCR bigezweho (cyane cyane ibikoresho byihuta kandi byoroshye), no guteza imbere PCR kuva muri laboratoire kugera kumurima mugari nko kuryama kwa clinique no gutahura aho.
TES1-15809T200 kumashini ya PCR
Ubushyuhe bwo ku ruhande bushyushye: 30 C,
Imax ： 9.2A ，
Umax: 18.6V
Qmax ： 99.5 W.
Delta T max ： 67 C.
ACR ： 1.7 ± 15% Ω （1.53 kugeza 1.87 Ohm）
Ingano ： 77 × 16.8 × 2.8mm
Igihe cyo kohereza: Kanama-13-2025
